sCD40 Ligand Antibody

Signal Transduction, Antibody Pairs

ELISA:

To detect hsCD40L by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsCD40L.

Sandwich:

To detect hsCD40-Ligand by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.

Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

P29965

959

CD40LG

Homo sapiens

To detect hsCD40-Ligand by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.

To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.