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LIGHT Antibody

542,00 €
Zzgl. MwSt.
ArtNr PRS-XP-5215-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Lyophilized
Applikationen WB, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias LTg, TR2, CD258, HVEML, LIGHT, UNQ391/PRO726, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L
Lieferbar
Shipping
blue ice or RT
By Research Area
Infectious Disease, Apoptosis, Chemokines & Cytokines, Herpes, Antibody Pairs
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
Applications
ELISA:

To detect hLIGHT by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLIGHT.

Sandwich:

To detect hLIGHT by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human LIGHT (XP-5215Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Western Blot:

To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification
Anti-hLIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix.
Clonality
Polyclonal
Conjugate
Unconjugated
Storage Conditions
LIGHT antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Disclaimer
This product is for research use only.
Modifications
None
Ncbi Official Symbol
TNFSF14
Accession #
O43557
Protein Gi #
313104028
Ncbi Gene Id #
8740
User Note
Centrifuge vial prior to opening.
Ncbi Official Symbol
TNFSF14
Ncbi Official Full Name
tumor necrosis factor (ligand) superfamily, member 14
Ncbi Organism
Homo sapiens
Swissprot #
O43557
Background
LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.
1st Image Caption
To detect hLIGHT by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human LIGHT (XP-5215Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.
2nd Image Caption
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3rd Image Caption
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
4th Image Caption
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 buffer is recommended. Optimal concentrations and conditions may vary.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
Listenpreis: 542,00 €
Preis: 542,00 €
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Lieferung vsl. bis 02.10.2025 

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