LAG3 Antibody [9F9]

Immunology

LAG-3 antibody can be used for ELISA starting at 0.25 μ g/mL. For Immunocytochemistry start at 1 μ g/mL. For Immunofluorescence start at 10 μ g/mL. For Immunohistochemistry start at 5 μ g/mL. For Flow Cytometry start at 1 μ g/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Cat. No. 1306 - Human Spleen Tissue

Monoclonal

IgG2a, k

LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

LAG-3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

NP_002277

3902

LAG3

Homo sapiens

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μ g/ml.

Green: LAG3 Antibody [9F9] (RF16084)
Blue: DAPI staining

Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μ g/ml.

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.