SARS-CoV-2 Spike P26S Antibody (Gamma Variant)

blue ice or RT

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)

WB: 1 μ g/mL.

Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.

SARS-CoV-2 Spike P26S (Gamma Variant) antibody is affinity chromatography purified via peptide column.

IgG

SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.

SARS-CoV-2 Spike P26S antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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1791269090

Optimal dilutions for each application to be determined by the researcher.

surface glycoprotein

In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named as Gamma variant, was discovered in Japan and later spread in Brazil. It is considered as VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.

Wang et al. Cell Host & Microbe 2021, 29(5): 747-751.

Figure 1 SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in ELISA
Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), and delta variant (B.1.617.2), 1 μ g/mL, incubate at 4 ˚ C overnight.Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr.

Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein
Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1).Detection Antibodies: Spike P26S (Gamma Variant) antibody, 9573, 1 μ g/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300).