SARS-CoV-2 (COVID-19) NSP8 Antibody

Infectious Disease, COVID-19

Anti-SARS-CoV-2 (COVID-19) NSP8 antibody (9159) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein.

The immunogen is located within 60-110 amino acids of SARS-CoV-2 (COVID-19) NSP8.

SARS-CoV-2 (COVID-19) NSP8 Antibody is affinity chromatography purified via peptide column.

IgG

SARS-CoV-2 (COVID-19) NSP8 Antibody is supplied in PBS containing 0.02% sodium azide.

SARS-CoV-2 (COVID-19) NSP8 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

YP_009742615.1

43740578

ORF1ab

Severe acute respiratory syndrome coronavirus 2

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP8 plays a role in viral RNA synthesis (3, 4, 5). Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, it may synthesize substantially longer products than oligonucleotide primers (6).

Hui et al. Int J Infect Dis. 2020; 91:264-266.

Yin et. al Science 2020; 0:0-0

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP8 Protein
Antibodies: SARS-CoV-2 (COVID-19) NSP8 Antibody, 9159. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (10-415) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL