GLS Antibody

645,00 €

Zzgl. MwSt.

ArtNr	PRS-63-652-200ul
Hersteller	ProSci
Menge	200 ul
Kategorie	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Immunostaining
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
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Shipping	blue ice or RT
By Research Area	Obesity, Neuroscience, Signal Transduction
Immunogen	This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Applications	For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:25 For IF starting dilution is: 1:25
Predicted Molecular Weight	73 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	GLS
Accession #	O94925
Ncbi Gene Id #	2744
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	GLS
Ncbi Official Full Name	Glutaminase kidney isoform, mitochondrial
Ncbi Organism	Homo sapiens
Swissprot #	O94925
Background	Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Background References 1	Swierczynski, J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993)
1st Image Caption	Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
2nd Image Caption	Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
3rd Image Caption	Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
4th Image Caption	Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
5th Image Caption	Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
6th Image Caption	Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody at 1:1000 at each lane.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 200 ul

Listenpreis: 645,00 €

Preis: 645,00 €

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