VIME Antibody

Cancer, Cell Cycle, Neuroscience, Signal Transduction

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P08670

7431

VIM

Homo sapiens

VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.

Western blot analysis of VIME Antibody in A375 cell line lysates (35ug/lane)

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.