ROR1 Antibody

684,00 €

Zzgl. MwSt.

ArtNr	PRS-63-129-200ul
Hersteller	ProSci
Menge	200 ul
Kategorie	Oncology Neuroscience Molecular Targets for Neuroscience Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies By Organ Colorectal Cancer Heart & Blood Cancer Immunostaining
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, FC, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Lieferbar
Shipping	blue ice or RT
By Research Area	Neuroscience, Signal Transduction
Immunogen	This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Applications	For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000
Predicted Molecular Weight	104 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ROR1
Accession #	Q01973
Protein Gi #	118572711
Ncbi Gene Id #	4919
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ROR1
Ncbi Official Full Name	Tyrosine-protein kinase transmembrane receptor ROR1
Ncbi Organism	Homo sapiens
Swissprot #	Q01973
Background	ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Background References 1	Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
Background References 2	Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
Background References 3	Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
Background References 4	Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).
1st Image Caption	Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2nd Image Caption	Antibody staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
3rd Image Caption	Antibody staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
4th Image Caption	Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
5th Image Caption	Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody at 1:1000 at each lane.
6th Image Caption	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 200 ul

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Preis: 684,00 €

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