EphA4 Antibody

Neuroscience, Signal Transduction

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P54764

2043

EPHA4

Homo sapiens

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using EPHA4 Antibody (R890) at 1:1000 at each lane.

Western blot analysis of lysates from Hela, NCI-H460, mouse NIH/3T3 cell line and human ovary tissue lysate (from left to right), using EPHA4 Antibody (R890) at 1:1000 at each lane.

Western blot analysis of EPHA4 using EphA4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).