PDK4 Antibody

645,00 €

Zzgl. MwSt.

ArtNr	PRS-62-612-200ul
Hersteller	ProSci
Menge	200 ul
Kategorie	Neuroscience Targets for Rodent Neurosciences Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Immunostaining
Typ	Antibody Primary
Format	Liquid
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4
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Shipping	blue ice or RT
By Research Area	Obesity, Signal Transduction
Immunogen	This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
Applications	For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IF starting dilution is: 1:100
Predicted Molecular Weight	46 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PDK4
Accession #	Q16654
Protein Gi #	3183120
Ncbi Gene Id #	5166
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PDK4
Ncbi Official Full Name	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Ncbi Organism	Homo sapiens
Swissprot #	Q16654
Background	PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Background References 1	References for protein:
Background References 2	Rosa, G., et al., Obes. Res. 11(2):176-182 (2003).
Background References 3	Razeghi, P., et al., Cardiology 97(4):203-209 (2002).
Background References 4	Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996).
1st Image Caption	Antibody staining PDK4 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
2nd Image Caption	Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
3rd Image Caption	Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
4th Image Caption	Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
5th Image Caption	Western Blot at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 ug per lane.
6th Image Caption	Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
7th Image Caption	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 200 ul

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Preis: 645,00 €

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