WNT4 Antibody

blue ice or RT

This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.

39 kDa

Polyclonal

Unconjugated

batch dependent

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

WNT4

20532425

Optimal dilutions for each application to be determined by the researcher.

Protein Wnt-4

P56705

Kuulasmaa, T., Horm. Metab. Res. 40 (10), 668-673 (2008)

Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lane 3: mouse bladder lysate Lane 4: rat lung lysate Lysates/proteins at 20 ug per lane.