WNT5A Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Rabbit

For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P41221

7474

WNT5A

Homo sapiens

The WNT gene family consists of structurally related geneswhich encode secreted signaling proteins. These proteins have beenimplicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning duringembryogenesis. This gene is a member of the WNT gene family. Itencodes a protein which shows 98%, 98% and 87% amino acid identityto the mouse, rat and the xenopus Wnt5A protein, respectively. Theexperiments performed in Xenopus laevis embryos identified thathuman frizzled-5 (hFz5) is the receptor for the Wnt5A ligand andthe Wnt5A/hFz5 signaling mediates axis induction. [provided byRefSeq].

Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995)

Clark, C.C., et al. Genomics 18(2):249-260(1993)

Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: PANC-1 whole cell lysate Lane 3: mouse heart lysate Lane 4: rat heart lysate Lysates/proteins at 20 ug per lane.

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.