DEPD5 Antibody

645,00 €

Zzgl. MwSt.

ArtNr	PRS-56-172-200ul
Hersteller	ProSci
Menge	200 ul
Kategorie	Oncology Antibodies Flow Cytometry Western Blot Primary Antibodies By Organ Bladder Cancer Testicular & Prostate Cancer Immunostaining
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, FC
Specific against	Human (Homo sapiens)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DEP domain-containing protein 5, DEPDC5, KIAA0645
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Shipping	blue ice or RT
By Research Area	Cancer, Neuroscience
Homology	Predicted species reactivity based on immunogen sequence: Mouse
Immunogen	This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids from the C-terminal region of human DEPD5.
Applications	For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000
Predicted Molecular Weight	181 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	DEPDC5
Accession #	O75140
Ncbi Gene Id #	9681
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	DEPDC5
Ncbi Official Full Name	DEP domain-containing protein 5
Ncbi Organism	Homo sapiens
Swissprot #	O75140
Background References 1	Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005)
Background References 2	Dunham, I., et al. Nature 402(6761):489-495(1999)
1st Image Caption	Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2nd Image Caption	Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
3rd Image Caption	Western blot analysis of DEPD5 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 200 ul

Listenpreis: 645,00 €

Preis: 645,00 €

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