IL-17 Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Bovine: (84%), Rat: (81%), Pig: (74%)

WB: 0.125-2 μ g/mL; IF: 5-20 μ g/mL; IHC: 1-5 μ g/mL .

Antibody validated: Western Blot in human samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.

Predicted: 17.5kD

Observed: 18 kDa

Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and Rat IL-17 also has one isoform (150aa, 16.9kD). 4887 can detect human, mouse and rat IL-17.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4887P – IL-17 Peptide

None

NP_002181

3605

IL17A

Homo sapiens

IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κ B and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

Paunovic et al. Rheumatol.2008; 47:771-6.

Figure 1 Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μ g/mLLane 2: 0.25 μ g/mLLane 3: 0.5 μ g/mL

Figure 3 Immunohistochemistry Validation of IL-17 in Human Lymph Node
Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17 antibody (4887) at 1 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 5 Immunofluorescence Validation of IL-17 in Mouse A-20 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17 with 4887 at 5 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 7 Immunohistochemistry Validation of IL-17 in Mouse Colon Tissue
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17 antibody (4887) at 2 μ g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.