TIM-3 Antibody

645,00 €

Zzgl. MwSt.

ArtNr	PRS-46-495-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Antibodies Western Blot ELISA Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, FC, IF, ELISA
Specific against	Human (Homo sapiens)
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3
Lieferbar
Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Dog
Immunogen	The immunogen for this antibody is: C-KWYSHSKEKIQN
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell lines Jurkat and MOLT-4 and in Human Tonsil lysates (calculated MW of 33.4kDa according to NP_116171.3). Recommended concentration:1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 (squamous carcinoma) cell lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HepG2 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Specificity	.
Positive Control 1	Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 46-495P - TIM-3 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HAVCR2
Accession #	NP_116171.3
Protein Gi #	49574534
Ncbi Gene Id #	84868
Ncbi Official Symbol	HAVCR2
Ncbi Official Full Name	hepatitis A virus cellular receptor 2
Ncbi Organism	Homo sapiens
Swissprot #	Q8TDQ0
Background References 1	Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases. Trends Mol Med. 2005 Aug; 11(8):362-9. Review.
1st Image Caption	46-495 (2ug/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2nd Image Caption	46-495 (1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
3rd Image Caption	46-495 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic and nuclear staining. The nu
4th Image Caption	46-495 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

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Menge: 0.1 mg

Listenpreis: 645,00 €

Preis: 645,00 €

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