Vimentin Antibody

645,00 €

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ArtNr	PRS-43-649-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development Antibodies Cell Biology Cytoskeleton Western Blot ELISA Primary Antibodies Intermediate filaments Stem Cell Markers
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, FC, IF, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	VIM, vimentin, FLJ36605
Lieferbar
Shipping	blue ice or RT
By Research Area	Homeostasis, Homeostasis
Homology	Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen	The immunogen for this antibody is: C-QVINETSQHHDDLE
Applications	Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Predicted Molecular Weight	Approx 55kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 43-649P - Vimentin Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	Vimentin
Accession #	NP_003371.2
Protein Gi #	55977767
Ncbi Gene Id #	7431
Ncbi Official Symbol	VIM
Ncbi Official Full Name	vimentin
Ncbi Organism	Homo sapiens
Swissprot #	P08670
Background References 1	Maraina CH, Nurdayana AK, Rusni D, Azwany Y.Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis.
1st Image Caption	43-649 (2ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	43-649 (0.1ug/ml) staining of Mouse (A) and (2ug/ml) Rat (B) Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
3rd Image Caption	Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed
4th Image Caption	43-649 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/Intermediate filament staining. The nuc

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Menge: 0.1 mg

Listenpreis: 645,00 €

Preis: 645,00 €

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