NEFM Antibody

ArtNr	PRS-42-197-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences Antibodies Western Blot ELISA Primary Antibodies Cytoskeleton Markers Schwann Cell Markers
Typ	Antibody Primary
Format	Liquid
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NEFM, neurofilament, medium polypeptide, NEF3, NF-M, NFM, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
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Shipping	blue ice or RT
By Research Area	Stem Cell , Neuroscience
Homology	Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen	The immunogen for this antibody is: C-QASHITVERKDYLK
Applications	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Specificity	This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
Positive Control 1	Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 42-197P - NEFM Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NEFM
Accession #	NP_005373.2
Protein Gi #	157738649
Ncbi Gene Id #	4741
Ncbi Official Symbol	NEFM
Ncbi Official Full Name	neurofilament, medium polypeptide
Ncbi Organism	Homo sapiens
Swissprot #	P07197
Background References 1	Levy E, Liem RK, D\1Eustachio P, Cowan NJ Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein European J. Biochem. 1987 Jul 166 (1): 71-7
1st Image Caption	42-197 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2nd Image Caption	42-197 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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Lieferung vsl. bis 07.08.2025