IL-33 Antibody [IL33305B]

blue ice or RT

Recombinant human IL-33.

Recognizes human IL-33. Does not cross-react with mouse IL-33.

Monoclonal

IgG2aκ

Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Stable for at least 1 year after receipt when stored at -20˚ C.

None

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B) (Prod. No. 36-355). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to thewells followed by washing; 3) add anti-FLAG HRP conjugated (1:2, 000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm

Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) (Prod. No. 36-355) at 1:2, 000 dilution. 1. Recombinant human IL-33 (His tagged). 2. Recombinant human IL-33 (FLAG®-tagged). 3. Recombinant mouse IL-33 (FLAG®-tagged). 4. Other protein (His tagged, negative control).