SARS-CoV-2 (COVID-19) Envelope Antibody (biotin)

blue ice or RT

Predicted reactivity based on immunogen sequence: SARS-CoV Envelope proteins: (100%)

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope has one isoform (75aa).

Polyclonal

Biotin

1 mg/mL

Cat. No. 3531P – SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Peptide

None

QHD43418

43740570

E

Wuhan seafood market pneumonia virus

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Envelope protein is a small polypeptide that contains at least one alpha-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication (3).

Hui et al. Int J Infect Dis. 2020; 91:264-266.

Nuovo GJ et al. Cytologic and molecular correlates of SARS-CoV-2 infection of the nasopharynx. Ann Diagn Pathol. 2020; 48:151565. doi: 10.1016/j.anndiagpath.2020.151565. PMID: 32659620

Nuovo G, Tili E, Suster D, Matys E, Hupp L, Magro C. Strong homology between SARS-CoV-2 envelope protein and a Mycobacterium sp. antigen allows rapid diagnosis of Mycobacterial infections and may provide specific anti-SARS-CoV-2 immunity via the BCG vaccine. Ann Diagn Pathol. 2020; 48:151600. doi: 10.1016/j.anndiagpath.2020.151600. PMID: 32805515

Magro, et al. Docked severe acute respiratory syndrome coronavirus 2 proteins within the cutaneous and subcutaneous microvasculature and their role in the pathogenesis of severe coronavirus disease 2019. Hum Pathol. 2020 Dec; 106:106-116. doi: 10.1016/j.humpath.2020.10.002. Epub 2020 Oct 12.PMID: 33058948

Mulvey, et al. Analysis of complement deposition and viral RNA in placentas of COVID-19 patients. Ann Diagn Pathol. 2020 Jun; 46:151530. doi: 10.1016/j.anndiagpath.2020.151530. Epub 2020 Apr 25.PMID: 32387855

Figure 1 ELISA Validation
Coating Antigen: immunogen peptide, 3531P, 10 μ g/mL, incubate at 4 ˚ C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3531-biotin or 3531, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3531-biotin was detected by HRP-conjugated streptavidin at 1:5, 000 and 3531 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10, 000 , incubate at RT for 1 hr.