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BrdU Antibody [SPM537]

BrdU Antibody [SPM537]

788,00 €

Zzgl. MwSt.

ArtNr	PRS-34-204-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Antibodies Cell Biology Cell Status Apoptosis Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	FC, IF, IHC
Specific against	other
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Lieferbar
Shipping	blue ice or RT
By Research Area	Cell Cycle
Immunogen	Bromodeoxyuridine was used as the immunogen for this anti-BrdU antibody.
Applications	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-BrdU antibody for each application should be determined by the researcher. 1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	SPM537
Isotype	IgG1
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
User Note	Optimal dilutions for each application to be determined by the researcher
Swissprot #	Not Applicable
Background	It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
1st Image Caption	Formalin-fixed, paraffin-embedded mouse small intestine stained with anti-BrdU antibody (SPM537).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

Listenpreis: 788,00 €

Preis: 788,00 €

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Lieferung vsl. bis 04.09.2025

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