« Zurück
Startseite /
BrdU Antibody [BRD494]

BrdU Antibody [BRD494]

788,00 €

Zzgl. MwSt.

ArtNr	PRS-33-238-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Antibodies Cell Biology Cell Status Apoptosis Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	FC, IF, IHC
Specific against	other
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Lieferbar
Shipping	blue ice or RT
By Research Area	Cell Cycle
Immunogen	BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
Applications	Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance. 1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	BRD494
Isotype	IgG1
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Gene Id #	Not Applicable
User Note	Optimal dilutions for each application to be determined by the researcher
Background	This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
1st Image Caption	IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD494).

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

Produkt-Datenblatt anfordern

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 100 ug

Listenpreis: 788,00 €

Preis: 788,00 €

lieferbar

Lieferung vsl. bis 04.09.2025

Anzahl:

Vergleichen