Estrogen Receptor Antibody [ER505]

Signal Transduction

WB: use antibody at 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titration of the antibody may be required for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Monoclonal

IgG1, kappa

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

None

2099

ESR1

homo sapiens

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. It is found in two forms, alpha and beta. Both forms are widely expressed, however, alpha is the predominant form in endometrium and breast cancer cells, and beta is more highly expressed in brain, kidney, heart, lung and bone. Activated Estrogen receptor dimerizes and forms both homo- and heterodimers. ER contains five domains: A-F. The A and B domain are able to weakly activate ER in the absence of ligand. The C domain binds DNA and the E domain contains the ligand binding site. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This antibody is specific to estrogen receptor alpha and shows minimal cross-reaction with other members of the family.

Western blot testing of human samples using Estrogen receptor antibody (ER505).