ACE2 Antibody

159,00 €

Zzgl. MwSt.

ArtNr	PRS-3217-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Kategorie	Oncology Neuroscience Aging & Neurological Disorders Neural Signaling Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Testicular & Prostate Cancer
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Lieferbar
Shipping	blue ice or RT
By Research Area	Infectious Disease
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen	Anti-ACE2 antibody (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
Applications	WB: 1-2 μ g/mL; IHC: 2 μ g/mL; IF: 10 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity	Anti-ACE2 has no cross response to ACE1.
Positive Control 1	Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2	Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3	Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4	Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight	Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation)
Validation	Independent Antibody Validation in Cell lines (Figure 1) shows similar ACE2 expression profile in human cell lines detected by three independent anti-ACE2 antibodies that recognize different epitopes, 3215 against central domain, 3217 against C-terminus domain and 3227 against N-terminus domain. ACE2 proteins are detected in the most tested tissues at different expression levels by three independent antibodies.
Isoforms	Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.
Purification	ACE2 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	ACE2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 3217P – ACE2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACE2
Accession #	NP_068576
Protein Gi #	11225609
Ncbi Gene Id #	59272
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACE2
Ncbi Official Full Name	angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYF1
Background	ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Background References 1	Donoghue et al. Circ. Res. 2000; 87:1-9.
Background References 2	Tipnis et al. J Biol. Chem. 2000; 275:33238-43.
Background References 3	Li et al. Nature 2003; 426:450-4.
Background References 4	Lu et al. The Lancet 2020 (published online).
Background References 5	Crackower et al. Nature 2002; 417:822-8.
Citation 1	Wei, et al.Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2020 Oct 20; S0092-8674(20)31392-1. doi: 10.1016/j.cell.2020.10.028.PMID: 33147444
1st Image Caption	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3215 (2 μ g/mL), ACE2, 3217 (2 μ g/mL), ACE2, 3217 (2 μ g/mL) and beta-actin 3779 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2nd Image Caption	Figure 2 Western Blot Validation in Human Tissues and Cell Line Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3217 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3rd Image Caption	Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3217 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
4th Image Caption	Figure 4 Western Blot Validation in Rat Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3217 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
5th Image Caption	Figure 5 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with 3217 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
6th Image Caption	Figure 6 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with 3217 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
7th Image Caption	Figure 7 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with 3217 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
8th Image Caption	Figure 8 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with 3217 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
9th Image Caption	Figure 9 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3217) at 2 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
10th Image Caption	Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with 3217 at 10 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
11th Image Caption	Figure 11 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with 3217 at 5 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

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Menge: 0.02 mg

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Preis: 159,00 €

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