MonoMethyl-Histone H3-K79 pAb

blue ice or RT

A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3

HeLa

Observed: 18kDa

Polyclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

Methylated

8290

H3K79me1

Homo sapiens

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K79 antibody (18-630).

Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K79 antibody (18-630) and rabbit IgG. P1 and P2 were located on GAPDH gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.