NEFM Antibody

ArtNr	PRS-16-591-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Antibodies Western Blot Immunofluorescence Primary Antibodies Schwann Cell Markers
Typ	Antibody Primary
Format	Liquid
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NEFM, neurofilament-3 (150 kD medium), neurofilament, medium poly peptide 150 kDa, neurofilament triplet M protein, NFM, NF-M, NEF3, medium poly peptide, NEFM
Lieferbar
Shipping	blue ice or RT
By Research Area	Cell Cycle, Neuroscience, Signal Transduction
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NEFM (NP_005373.2).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	MCF7
Positive Control 2	rat brain
Predicted Molecular Weight	Observed: 140kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NEFM
Ncbi Gene Id #	4741
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	NEFM
Ncbi Official Full Name	neurofilament, medium polypeptide
Ncbi Organism	Homo sapiens
Swissprot #	P07197
Background	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
1st Image Caption	Western blot analysis of extracts of various cell lines, using NEFM antibody (16-591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
2nd Image Caption	Immunofluorescence analysis of mouse brain using NEFM antibody (16-591) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of rat brain using NEFM antibody (16-591) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

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Lieferung vsl. bis 07.08.2025