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KRT14 Antibody

451,00 €
Zzgl. MwSt.
ArtNr PRS-15-857-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias KRT14, K14, NFJ, CK14, EBS3, EBS4
Lieferbar
Shipping
blue ice or RT
By Research Area
Cell Cycle, Signal Transduction
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Positive Control 1
mouse brain
Positive Control 2
mouse heart
Positive Control 3
mouse liver
Predicted Molecular Weight
Observed: 56kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
KRT14
Ncbi Gene Id #
3861
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
KRT14
Ncbi Official Full Name
keratin 14
Ncbi Organism
Homo sapiens
Swissprot #
P02533
Background
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
1st Image Caption
Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
4th Image Caption
Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
5th Image Caption
Immunofluorescence analysis of Rat skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
6th Image Caption
Immunofluorescence analysis of Human skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
7th Image Caption
Immunofluorescence analysis of Mouse skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
Listenpreis: 451,00 €
Preis: 451,00 €
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Lieferung vsl. bis 04.09.2025 

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