BDNF Antibody

451,00 €

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ArtNr	PRS-14-776-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Antibodies Obesity Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Neuronal Cell Markers
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	BDNF , brain-derived neurotrophic factor, MGC34632, neurotrophin
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Shipping	blue ice or RT
By Research Area	Apoptosis, Cell Cycle, Growth Factors, Neuroscience, Obesity
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (NP_733928.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	A-549
Positive Control 2	SW480
Positive Control 3	Mouse kidney
Positive Control 4	Mouse brain
Positive Control 5	Rat brain
Predicted Molecular Weight	Observed: 14kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	BDNF
Ncbi Gene Id #	627
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	BDNF
Ncbi Official Full Name	brain-derived neurotrophic factor
Ncbi Organism	Homo sapiens
Swissprot #	P23560
Background	This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
1st Image Caption	Western blot analysis of extracts of various cell lines, using BDNF antibody (14-776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
2nd Image Caption	Immunofluorescence analysis of U2OS cells using BDNF antibody (14-776) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of U-251MG cells using BDNF antibody (14-776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

Listenpreis: 451,00 €

Preis: 451,00 €

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