GPER1 Antibody

451,00 €

Zzgl. MwSt.

ArtNr	PRS-13-509-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Oncology Neuroscience Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Antibodies By Cell Type Carcinoma Sarcoma Obesity Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Other Lipid\Cholesterol Metabolism Modulators
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, GPCR-Br, IL8-related receptor DRY12, Lymphocyte-der
Lieferbar
Shipping	blue ice or RT
By Research Area	Neuroscience, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human GPER1 (NP_001496.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	Mouse brain
Positive Control 2	Mouse heart
Positive Control 3	Rat brain
Predicted Molecular Weight	Observed: 52kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	GPER1
Ncbi Gene Id #	2852
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	GPER1
Ncbi Official Full Name	G-protein coupled estrogen receptor 1
Ncbi Organism	Homo sapiens
Swissprot #	Q99527
Background	This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3, 4, 5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
1st Image Caption	Western blot analysis of extracts of various cell lines, using GPER1 antibody (13-509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
2nd Image Caption	Immunofluorescence analysis of C6 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
4th Image Caption	Immunofluorescence analysis of U2OS cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

Listenpreis: 451,00 €

Preis: 451,00 €

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