p53 Antibody

blue ice or RT

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human p53 (NP_000537.3).

MCF7

Observed: 53kDa

Polyclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

None

7157

TP53

Homo sapiens

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 antibody (13-113) at dilution of 1:200 (40x lens).

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (13-113) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.