Anti-Mouse CD172a - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse brain membrane protein

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2831653

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this P84 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
CyTOF®

Other Applications Reported In Literature ?

IHC FF
IP
B

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone P84 recognizes an epitope on mouse CD172a.

Antigen Distribution

CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.

Background

CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)¹. SIRPα is expressed predominantly in myeloid cells² - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells³. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])⁴, is expressed in most cell types⁵. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells^{6, 7}. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation^8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages¹¹. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics¹².

Antigen Details

Protein

CD172a

Ligand/Receptor

CD47, SP-A, SP-D

Function

Negative regulation of several biological processes

PubMed

CD172a

NCBI Gene Bank ID

19261

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

.

Innate Immunity

.

Neuroscience

References & Citations

1. Fujioka, Y., et al. (1996) Mol. Cell. Biol. 16:6887

2. Adams, S., et al. (1998) J. Immunol. 161:1853

3. Chuang W, et al. (1990) Dev Biol. 137:219–232

4. Seiffert, M., et al. (1999) Blood 94:3633

5. Oldenborg P. A. (2013) ISRN Hematol. 2013:614619

6. Oldenborg, P. A., et al. (2000) Science 288:2051

7. Oldenborg, P. A., et al. (2001) J. Exp. Med. 193:855

8. Brooke, G. P., et al. (1998) Eur. J. Immunol. 28:1

9. Seiffert, M., et al. (2001) Blood 97:2741

10. Latour, S., et al. (2001) J. Immunol. 167:2547

11. Jaiswal S, et al. (2009) Cell. 138(2):271-85

12. Jalil AR, et al. (2020) Antib Ther. 3(2):80-94

Technical Protocols