Anti-Mouse Ly-6G - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse Ly-6G transfected EL-4J cell line

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737551

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

CyTOF®
Depletion
IHC (Frozen)
IHC (Paraffin)
WB

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.

Antigen Distribution

Ly6G is expressed by neutrophils.

Background

Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein¹. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds². Ly6G is expressed by murine neutrophils regardless of location and activation^{1, 4, 5}. Eosinophils may also express low levels of Ly6G⁵. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia⁶. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism⁷.

Antigen Details

Protein

Ly-6G

PubMed

Ly-6G

NCBI Gene Bank ID

546644

UniProt.org

Information on Uniprot.org

Research Area

Immunology

.

Innate Immunity

References & Citations

1.) Fleming TJ, et al. (1993) J Immunol. 151(5):2399-408
2.) Tsetlin VI. et al. (2015) Trends Pharmacol Sci. 36(2):109-23
3.) Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4.) Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5.) Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6.) Stroncek DF. et al. (2007) Curr Opin Hematol. 14(6):688-93
7.) Wang JX, et al. (2012) Blood. 120(7):1489-1498
8.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link

Technical Protocols