Anti-Human ICOS

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human ICOS (> 98%)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830733

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human ICOS this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human ICOS is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-551.

Other Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Inducible Costimulator (ICOS) recognizes Human ICOS. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Inducible co-stimulator (ICOS) is an inducible homodimeric costimulator protein that is structurally and functionally related to CD28. It is a third member of the CD28/CTLA4 family and it is expressed on activated T cells.¹ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses.² ICOS is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.³

Antigen Details

Protein

ICOS

PubMed

ICOS

NCBI Gene Bank ID

29851

UniProt.org

More Information on Uniprot.org

References & Citations

1. Flavell, RA. et al. (2001) Nature 409: 97

2. Bachmann, MF. et al. (2000) J Exp Med. 192: 53

3. Kroczek, RA. et al. (1999) Nature 397: 263

Technical Protocols