Anti-Mouse IFNgamma (Clone H22) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

Purified Recombinant Mouse IFN-γ (> 98%)

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Ambient

RRID

AB_2737542

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: For Western blotting, the suggested use of clone H22 antibody is 0.6 µg/ml., (See Image Above).

Additional Reported Applications For Relevant Conjugates ?

N
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Armenian Hamster Anti-Mouse Interferon-Gamma (IFN-γ) (Clone H22) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.⁴ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.⁵ IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.⁶

Antigen Details

Protein

Interferon Gamma

Ligand/Receptor

IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)

PubMed

Interferon Gamma

NCBI Gene Bank ID

15978/a>

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2. Diamond, MS. et al. (2017) J Virol. 91(22): e01419-17. PubMed
3. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
4. Goeddel, DV. et al. (1982) Nature 298: 859
5. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
6. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163

Technical Protocols