Anti-Mouse IL-1beta - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

E. coli -expressed, recombinant mouse IL-1β

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Ambient

RRID

AB_2737541

Additional Reported Applications For Relevant Conjugates ?

N
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Armenian Hamster Anti-Mouse IL-1β (Clone B122) recognizes Mouse IL-1β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells and many other B cells, T cells, related monocytes

Background

Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes.^{1, 2} IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease.³. The B122 antibody has been reported to neutralize the bioactivity of natural or recombinant IL-1β.

Antigen Details

Protein

Interleukin-1 Beta

Function

Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, and glioblastoma cells

PubMed

Interleukin-1 Beta

NCBI Gene Bank ID

16176/a>

UniProt.org

Information on Uniprot.org

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Goldberg, Diamond, et. al (2017); PLoS ONE 12(5): e0177142 Article Link
1. Dinarello CA et al. (1996) Blood 87: 2095
2. Yuan et al. (1993) Cell 75: 641
3. Shin Maeda et al. (2005) Science 4: 735

Technical Protocols