Anti-Mouse IFNgamma (Clone H22) - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2830520

Applications and Recommended Usage?
Quality Tested by Leinco

WB The suggested concentration for this clone H22 antibody for use in western blotting is 0.5 μg/ml.

Other Applications Reported In Literature ?

N
ELISA
IF
IP
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Armenian Hamster Anti-Mouse Interferon Gamma (IFN-γ) (Clone H22) recognizes an epitope on Mouse IFN-γ. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.

Background

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.³ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.⁴ IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.⁵

Antigen Details

Protein

IFN-γ

Ligand/Receptor

IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)

PubMed

IFN-γ

NCBI Gene Bank ID

15978

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed

2.) Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed

3.) Goeddel, DV. et al. (1982) Nature 298: 859

4.) Wilson, CB. et al. (2007) Adv. Immunol. 96: 41

5.) Hume, DA. et al. (2004) J Leukoc Biol. 75: 163

6.) Karki et al. (2021) Cell. 184:149–168 Journal Link

Technical Protocols