Anti-Human CD71 - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2829683

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone T56/14 recognizes human CD71.

Antigen Distribution

CD71 is expressed on most proliferating cells, activated lymphocytes, monocytes, macrophages, erythroid progenitors, and brain endothelium.

Background

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein. The function of CD71 is believed to be primarily nutritional. It plays a role in the control of cellular proliferation through facilitation of iron uptake by way of ferrotransferrin binding and the recycling of apotransferrin to the cell surface. Additionally, transferrin receptor is required for erythropoiesis and proper neurological development, and it has also been suggested that a growth signal might be generated by the transferrin/transferrin receptor interaction. Current genetic analysis indicates that the structural genes for transferrin receptor and for a melanoma-associated antigen (p97), and perhaps transferrin itself, each map to a common chromosome in humans. These proteins exhibit primary sequence homology with transferrin and have the ability to bind ferric iron. Therefore, it is thought that genetic rearrangements in this iron transport region may be associated with malignant transformation. Hence, anti-CD71 mAbs are thought to have therapeutic potential in cases of human leukemia & lymphoma.

Antigen Details

Protein

CD71

Ligand/Receptor

Transferrin

PubMed

CD71

NCBI Gene Bank ID

7037

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. Pessin, JE. et al. (2003) J Biol Chem. 278(12):10683-90. Article Link
2. Trowbridge, SI. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
3. Iacopetta, BJ. et al. (1983) J. Histochem. Cytochem. 31:336

Technical Protocols