Anti-Mouse CD4 (Clone YTS 191) - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Unknown

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2893557

Applications and Recommended Usage?
Quality Tested by Leinco

FC

Other Applications Reported In Literature ?

Depletion
IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone YTS 191 recognizes an epitope on mouse CD4.

Antigen Distribution

CD4 is expressed on most thymocytes, MHC class II-restricted T cells, a subset of NKT cells, and subsets of dendritic cells and macrophages.

Background

CD4 antibody, clone YTS 191, recognizes CD4, a 58 kDa type I transmembrane glycoprotein of the Ig superfamily. CD4 is expressed by the majority of thymocytes, MHC class II-restricted T cells (helper T cells and immunosuppressive regulatory T cells), and subsets of natural killer T (NKT) cells, dendritic cells (DCs) and macrophages^1-3. On T cells, CD4 is a co-receptor of the T cell receptor (TCR) and interacts with major histocompatibility complex (MHC) II molecules on antigen-presenting cells (APCs). CD4 contributes to T cell development and selection and enhances TCR-dependent signaling by up to 100-fold through the accumulation of Lck^4-6. CD4 also contributes to the activation of NKT, macrophages, and DCs^{7, 8}.

Antigen Details

Protein

CD4

PubMed

CD4

Research Area

Immunology

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Esashi E, et al. (2003) J Immunol. 171(6):2773-7
3. Vremec D, et al. (2000) J Immunol. 164(6):2978-86
4. Li QJ, et al. (2004) Nat Immunol. 5:791–9
5. Janeway C. A. Jr. (1992) Annu Rev Immunol. 10:645–74
6. Germain RN. (2002) Nat Rev Immunol. 2(5):309-22
7. Thedrez A, et al. (2007) Blood. 110(1):251-8
8. Gibbings D & Befus AD. (2009) J Leukoc Biol. 86(2):251-9

Technical Protocols