Anti-Human CD57 (Clone VC1.1) - Biotin

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

0.5 mg/ml

Formulation

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829618

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD57 (Clone VC1.1) recognizes Human CD57. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-CD57 recognizes a (Mr 110 kD) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).¹ This antibody blocks binding of HNK-1 (Leu. 7) antibody.³

Antigen Distribution

The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.

Background

Anti-CD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and T-lymphocyte subsets, studies of neural tissue, malignant tissues and AIDS-related complex.

Antigen Details

Protein

CD57

Ligand/Receptor

Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.

PubMed

CD57

NCBI Gene Bank ID

27087

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Barnstable, C. et al. (1995) Brain Research 559:118
2. Leukocyte Typing V (1995) Oxford University Press: Code No. NK67
3. Arimatsu, Y. et al. (1987) J. Neuroscience 7:1250
4. Abo, T., and Balch, C.M. et al. (1981) J. Immunol. 127:1024

Technical Protocols