Anti-Mouse 4-1BB - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Recombinant Mouse CD137 human Fc fusion protein

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737470

Applications and Recommended Usage?
Quality Tested by Leinco

WB
ELISA

Other Applications Reported In Literature ?

in vivo 4-1BB stimulation
in vitro 4-1BB stimulation

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 3H3 recognizes an epitope on mouse 4-1BB.

Antigen Distribution

4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells.

Background

4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-B, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic anti-CD137 antibodies have been reported to stimulate a more intense immune system attack on cancers.

Antigen Details

Protein

4-1BB

Ligand/Receptor

4-1BB (CDw137)

PubMed

4-1BB

NCBI Gene Bank ID

21942

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Shan B et al. (2008) Cell Mol Immunol. 5: 379
2. Kwan BS et al. (1996) Cell Immunol. 169: 91
3. Kwan BS et al. (1998) Eur J Immunol. 28: 881
4. Kwan BS et al. (2009) J Immunol. 182: 4107

Technical Protocols