Anti-Mouse CD278 (ICOS) - PE

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse ICOS cDNA and ICOS hexahistidine fusion protein

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2829350

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 7E.17G9 recognizes an epitope on mouse CD278.

Antigen Distribution

ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages.

Background

CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases.

Antigen Details

Protein

CD278

Ligand/Receptor

B7h/B7RP-1/GL-50

PubMed

CD278

NCBI Gene Bank ID

54167

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Flavell, R. A. et al. (2001) Nature 409:97
2. Bachmann, M. F. et al. (2000) J. Exp. Med. 192:53
3. Kroczek, R. A. et al. (1999) Nature 397:263

Technical Protocols