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Bulk anti-Human CD3 Antibody (UCHT-1)

Bulk anti-Human CD3 Antibody (UCHT-1)

2.849,00 €

Zzgl. MwSt.

ArtNr	ICH1002-100MG
Hersteller	IchorBio
Menge	100mg
Kategorie	Antibodies In Vivo Antibodies Flow Cytometry Western Blot
Typ	Antibody
Applikationen	WB, FC, IP, IHC, FA
Clon	UCHT-1
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain
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Specificity	Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 a
Manufacturers Product Category	Low Endotoxin, anti-human
Target Information	CD3
Isotype Information	Mouse IgG1
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
Background	Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
Immunogen	Unknown
Concentration	≥ 2.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin	<1.0 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 5%
Storage	anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes	Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100mg

Listenpreis: 2.849,00 €

Preis: 2.849,00 €

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