Anti-Affinity Purified RAT IgG (H&L)(GOAT) (Min X MOUSE Serum Proteins) Secondary Antibody

Goat Polyclonal, Unconjugated

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) 1:1, 000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.

1:10, 000 - 1:40, 000

6.29 mg/mL by UV absorbance at 280 nm

ELISA 1:20, 000-1:110, 000; Western Blot 1:10, 000-1:40, 000; Immunochemistry 1:5, 000-1:20, 000

Rat

IgG (H&L)

None

Wet Ice

Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.