Goat anti-NEFM / NF-M Antibody

Goat anti-NEFM / NF-M Antibody
Principle Name: NEFM / NF-M
Official Symbol: NEFM
All Name and Symbols: NEFM; neurofilament, medium polypeptide; NEF3; NF-M; NFM; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium)
Accession Number(s): NP_005373.2
Peptide Sequence: QASHITVERKDYLK, is from internal region
Human Gene ID(s): 4741
Non-Human GeneID(s): 18040 (mouse); 24588 (rat);
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended co
Reference(s): Levy E, Liem RK, D'Eustachio P, Cowan NJ Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein European J. Biochem. 1987 Jul 166 (1): 71-7.PMID: 3036526