Goat anti-HRASLS3 / HREV107-3 Antibody

Goat anti-HRASLS3 / HREV107-3 Antibody
Principle Name: HRASLS3 / HREV107-3
Official Symbol: PLA2G16
All Name and Symbols: PLA2G16; phospholipase A2, group XVI; AdPLA; H-REV107-1; HRASLS3; HREV107; HREV107-3; MGC118754; HRAS-like suppressor 3; adipose-specific PLA2
Accession Number(s): NP_009000.2
Peptide Sequence: RAPIPEPKPGDLIE, is from N Terminus
Human Gene ID(s): 11145
Non-Human GeneID(s): 225845 (mouse); 24913 (rat);
Important Comments: Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0k
Reference(s): Nazarenko I, Kristiansen G, Fonfara S, Guenther R, Gieseler C, Kemmner W, Schafer R, Petersen I, Sers C. H-REV107-1 stimulates growth in non-small cell lung carcinomas via the activation of mitogenic signaling. Am J Pathol. 2006 Oct; 169(4):1427-39..PMID: 17003497