Goat anti-HRASLS2 Antibody

Goat anti-HRASLS2 Antibody
Principle Name: HRASLS2
Official Symbol: HRASLS2
All Name and Symbols: HRASLS2 ; HRAS-like suppressor 2; FLJ20556
Accession Number(s): NP_060348.1
Peptide Sequence: QELPYSLTSDNC, is from internal region
Human Gene ID(s): 54979
Non-Human GeneID(s):
Important Comments: This design was based on provisional sequence data.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17
Immunohistochemistry: In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml.
Reference(s): Shyu RY, Hsieh YC, Tsai FM, Wu CC, Jiang SY. Cloning and functional characterization of the HRASLS2 gene. Amino Acids. 2008 Jun; 35(1):129-37..PMID: 18163183