Goat anti-NET1 / ARHGEF8 (C Terminus) Antibody

Goat anti-NET1 / ARHGEF8 (C Terminus) Antibody
Principle Name: NET1 / ARHGEF8 (C Terminus)
Official Symbol: NET1
All Name and Symbols: NET1; ARHGEF8; NET1A; neuroepithelial cell transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelial cell transforming 1; RP11-116G8.1; p65 Net1 proto-oncogene protein; small GTP
Accession Number(s): NP_001040625.1; NP_005854.2
Peptide Sequence: DKALSGGKRKETLV, is from C Terminus
Human Gene ID(s): 10276
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave bands at approx 85kDa in Human Placenta and a very strong band at approx 40-50kDa in Human Skeletal Muscle lysates after 0.1ug/ml staining. Please note that currently we cannot find an explanation in the literat
Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration: 2.5ug/ml.
Reference(s): Chan AM, Takai S, Yamada K, Miki T. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. Oncogene. 1996 Mar 21; 12(6):1259-66..PMID: 8649828