Goat anti-GRAP2 / GRID / Grf40 Antibody

Goat anti-GRAP2 / GRID / Grf40 Antibody
Principle Name: GRAP2 / GRID / Grf40
Official Symbol: GRAP2
All Name and Symbols: GRAP2; GRID; P38; GADS; GRPL; GrbX; Mona; GRB2L; GRBLG; GRAP-2; GRB2-related adaptor protein 2; GRB-2-like protein; SH3-SH2-SH3 adaptor molecule; growth factor receptor-binding protein; GRB2-related protein with insert domain; hematopoietic cell-associate
Accession Number(s): NP_004801.1
Peptide Sequence: GLFPANYVAPMTR, is from C Terminus
Human Gene ID(s): 9402
Non-Human GeneID(s): 17444 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfe
Reference(s): Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M. Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1. Biochem Biophys Res Commun. 1998 Dec 18; 253(2):443-7..PMID: 9878555