Vimentin

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Optimal dilution should be determined by user

VIM, RP11-124N14.1, CTRCT30

When reconstituted with 175ul H20 dist, a final solution volume of 250 ul will contain 30mM Tris/HCl, pH 9.8, 9.5 M urea, 2mM DTT, 2 mM EDTA, 10 mM Methylammonium chloride with a protein concentration of 1mg/ml (according to protein dry weight)

Escherichia coli

pI 5.3