Anti-IgG4 (pFc subclass specific), HRP conjugate

The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1, 000 upwards. These data should be interpreted as general recommendations only.

Peroxidase conjugated purified monoclonal Mouse antibody to Human IgG4, subclass specific.

Highly purified monoclonal IgG4 isolated from pooled human serum

For Research Use Only.