Rat Thyrotropin-releasing hormone (TRH) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rat Thyrotropin-releasing hormone microplate
Rat Thyrotropin-releasing hormone standard
Rat Thyrotropin-releasing hormone detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Thyrotropin-releasing hormone (TRH) acts as a stimulator of hormone secretion from adenohypophyseal cells, and its signals are inactivated by TRH-degrading ectoenzyme.
The deduced 1, 024-amino acid type II integral transmembrane protein is 96% identical to the rat protein. It has 12 putative C-terminal N-glycosylation sites, a potential tyrosine sulfation site (residue 380), a consensus sequence for zinc-dependent metallopeptidases, and a short N-terminal intracellular domain with a potential phosphorylation site. Northern blot analysis revealed expression of 7.5-, 7.0-, 6.0-, and 4.5-kb transcripts. Strongest expression was in brain, with weaker expression in heart, lung, liver, and skeletal muscle, and no expression in kidney and placenta.

TRH; MGC125964; MGC125965;